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Inhibition of KIF20A by transcription factor IRF6 affects the progression of renal clear cell carcinoma

Xinwei Ma1,2, Xiaoqi Wang3, Qian Dong2

  • 1Department of Radiology, The Second Affiliated Hospital of Soochow University, No.1055 Sanxiang Road, Suzhou, 215004, Jiangsu, China.

Cancer Cell International
|May 4, 2021
PubMed
Abstract

Insights

Interferon regulatory factor 6 (IRF6) is downregulated in renal clear cell carcinoma (ccRCC), inhibiting tumor progression. Its target, KIF20A, is upregulated and promotes ccRCC cell proliferation and invasion.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Renal clear cell carcinoma (ccRCC) is a prevalent malignancy with increasing incidence.
  • The role of Interferon Regulatory Factor 6 (IRF6) in ccRCC remains uncharacterized despite its known involvement in tumorigenesis.

Purpose of the Study:

  • To investigate the expression and function of IRF6 in ccRCC.
  • To explore the relationship between IRF6, KIF20A, and ccRCC progression.
  • To elucidate the underlying molecular mechanisms of IRF6 in ccRCC.

Main Methods:

  • Bioinformatic analysis using GEPIA and HAP databases for gene expression and survival correlation.
  • Promoter analysis using JASPAR database.
  • Experimental validation including luciferase and ChIP assays.
  • In vitro and in vivo studies assessing ccRCC cell proliferation, invasion, and apoptosis.

Main Results:

  • IRF6 expression is downregulated in ccRCC tissues and correlates with poor prognosis.
  • IRF6 overexpression suppresses ccRCC cell proliferation, invasion, and migration.
  • KIF20A expression is upregulated in ccRCC tissues and associated with adverse outcomes.
  • KIF20A knockdown inhibits ccRCC cell proliferation, invasion, and migration.
  • KIF20A is a functional target of IRF6, partially mediating IRF6's effects on ccRCC cells.

Conclusions:

  • IRF6 acts as a tumor suppressor in ccRCC by inhibiting cell proliferation, invasion, and migration.
  • KIF20A is a key downstream target of IRF6 in ccRCC.
  • The IRF6/KIF20A pathway represents a potential therapeutic target for ccRCC.
Keywords:
Early diagnostic targetIRF6KIF20APrognosisRenal clear cell carcinoma

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