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Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction
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Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

[Interaction of aldolase A with lecithin liposomes]

A I Sytnik, Iu V Chumachenko, Z N Volovik

    Ukrainskii Biokhimicheskii Zhurnal (1978)
    |March 1, 1988
    PubMed

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    Overview
    Base-Catalyzed Aldol Addition Reaction01:08

    Base-Catalyzed Aldol Addition Reaction

    As depicted in Figure 1, base-catalyzed aldol addition involves adding two carbonyl compounds in aqueous sodium hydroxide to form a β-hydroxy carbonyl compound.
    Dehydration of Aldols to Enals: Base-Catalyzed Aldol Condensation01:14

    Dehydration of Aldols to Enals: Base-Catalyzed Aldol Condensation

    This lesson delves into the aldol condensation catalyzed by bases, where aldols undergo dehydration to enals. As shown in Figure 1, the β-hydroxy aldehyde formed in a base-catalyzed aldol addition reaction dehydrates on heating to yield an unsaturated carbonyl product, which is commonly referred to as an enal.
    Crossed Aldol Reaction Using Weak Bases01:14

    Crossed Aldol Reaction Using Weak Bases

    This lesson deals with the crossed aldol reaction using weak bases. The self-condensation of an aldehyde having α hydrogen is prevented by adding it slowly to a mixture of formaldehyde and weak bases like hydroxide and alkoxide. Upon slow addition of the aldehyde, the base deprotonates the α carbon of the aldehyde to form the corresponding enolate. The enolate subsequently attacks the formaldehyde to form a single crossed product. Figure 1 depicts the aforementioned reaction.
    Crossed Aldol Reaction Using Strong Bases: Directed Aldol Reaction00:56

    Crossed Aldol Reaction Using Strong Bases: Directed Aldol Reaction

    The reaction between two different carbonyl compounds comprising α hydrogen in the presence of a strong base like lithium diisopropylamide (LDA) to form a crossed aldol product is known as a directed aldol reaction. The directed aldol reaction is depicted in Figure 1.
    Acid-Catalyzed Aldol Addition Reaction01:15

    Acid-Catalyzed Aldol Addition Reaction

    The aldol reaction of a ketone under acidic conditions successfully forms an unsaturated carbonyl as the final product instead of an aldol. The acid-catalyzed aldol reaction is depicted in Figure 1.