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Related Experiment Video

Updated: Nov 7, 2025

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
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Cryomicroneedles for transdermal cell delivery.

Hao Chang1,2, Sharon W T Chew2, Mengjia Zheng2

  • 1Department of Biomedical Engineering, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China.

Nature Biomedical Engineering
|May 4, 2021
PubMed
Summary
This summary is machine-generated.

Cryogenic microneedle patches offer a simple, safe method for transdermal cell delivery, improving patient compliance and reducing risks. This technology successfully delivered viable cells, enhancing immune responses and slowing tumor growth in preclinical models.

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Area of Science:

  • Biotechnology
  • Dermatology
  • Immunology

Background:

  • Transdermal cell therapy requires innovative delivery systems beyond traditional injections.
  • Hypodermic injections for cell delivery face challenges like poor compliance, infection risk, and need for trained personnel.

Purpose of the Study:

  • To develop and demonstrate the efficacy of cryogenic microneedle patches for transdermal delivery of living therapeutic cells.
  • To evaluate the safety, viability, and therapeutic potential of cells delivered via microneedles.

Main Methods:

  • Fabrication of microneedles using stepwise cryogenic micromoulding with pre-suspended cells.
  • In vitro testing on porcine skin for insertion and dissolution properties.
  • In vivo studies in mice, including melanoma tumor models, comparing cryomicroneedle delivery with conventional injection methods.

Main Results:

  • Cryogenic microneedles successfully inserted into porcine skin and dissolved post-deployment.
  • Delivered cells maintained viability and proliferative capacity in mice.
  • Enhanced antigen-specific immune responses and slower tumor growth observed with cryomicroneedle delivery of dendritic cells compared to injections.

Conclusions:

  • Biocompatible cryogenic microneedle patches provide a minimally invasive and effective platform for transdermal cell delivery.
  • This technology holds promise for advancing various cell-based therapies, particularly in dermatology and oncology.
  • Cryomicroneedle patches represent a significant improvement over conventional cell injection methods.