Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

HoST: integrating Heuristic knowledge with attention-based LSTM networks for Thunderstorm prediction.

Scientific reports·2026

Same author

K<sub>3</sub>Ti<sub>2</sub>Cl<sub>9-<i>x</i></sub> Br <sub><i>x</i></sub> : structurally stable lead-free perovskites as permissive absorbers for solar cell and visible-light photocatalysis.

RSC advances·2026

Same author

Realistic benchmark RBD360 dataset for quality assessment of random user generated 360° videos.

Scientific reports·2026

Same author

BC-SwinNet: Swin transformer and CNN with multi-objective optimization for multi-class breast cancer detection using Histopathological images.

Scientific reports·2026

Same author

Explainable artificial-intelligence-based hyperspectral image analysis for leaf disease detection in intercropping system.

Frontiers in plant science·2026

Same author

A day sleep promoting role of phototransduction in <i>Drosophila melanogaster</i>.

Neurobiology of sleep and circadian rhythms·2026

Search research articles

Related Experiment Video

Updated: Nov 7, 2025

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

CRISPR Cas System: An efficient tool for cancer modelling.

Mehran Akhtar¹, Tazkira Jamal¹, Mudassir Khan²

¹Department of Biotechnology, Abdul Wali Khan University, Mardan, Pakistan.

JPMA. the Journal of the Pakistan Medical Association

|May 4, 2021

Summary

This summary is machine-generated.

The Clustered Regularly Interspaced Short Palindromic Repeats-Cas-9 (CRISPR-Cas9) system offers powerful genome editing for cancer genetics research. This review highlights its potential in understanding cancer biology and genetic modeling, though further work is needed for therapeutic applications.

Keywords:

CRISPR-Cas9, Cancer, Cancer modelling, Cancer genetics.

More Related Videos

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

Related Experiment Videos

Last Updated: Nov 7, 2025

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

Area of Science:

Molecular Genetics
Genomics
Cancer Research

Background:

The Clustered Regularly Interspaced Short Palindromic Repeats-Cas-9 (CRISPR-Cas9) system is a revolutionary tool for genome editing.
Cancer is a complex multi-gene disease requiring detailed genetic investigation.

Purpose of the Study:

To critically analyze and discuss the applications and outcomes of CRISPR-Cas9 technology in cancer genetics.
To evaluate the potential of CRISPR-Cas9 in understanding cancer biology and developing genetic models.

Main Methods:

A comprehensive narrative review of 50 research articles.
Critical analysis of CRISPR-Cas9 applications in cancer genetics.

Main Results:

CRISPR-Cas9 demonstrates significant potential for understanding various cancer types.
The system can be effectively utilized for creating genetic models of cancer.

Conclusions:

CRISPR-Cas9 holds great promise for advancing cancer genetics research and modeling.
Further research is essential to fully leverage CRISPR-Cas9 for elucidating cancer mechanisms and designing therapies.