Related Experiment Video
Updated: Nov 6, 2025

08:29
Mouse Epidermal Neural Crest Stem Cell EPI-NCSC Cultures
Published on: May 9, 2008
11.7K
Transient Induction and Characterization of Mouse Epiblast-Like Cells from Mouse Embryonic Stem Cells
Federico Pecori1, Shoko Nishihara2,3
1Laboratory of Cell Biology, Department of Bioinformatics, Graduate School of Engineering, Soka University, Tokyo, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|May 4, 2021
Abstract:
Mouse embryonic stem cells (mESCs) and mouse epiblast-like cells (mEpiLCs) recapitulate in vitro the epiblast first cell lineage decision, providing a powerful tool to investigate the mechanisms underlying the pluripotent state transition. Here, we describe a defined and robust protocol to transiently induce mEpiLCs from mESCs, together with a concise overview for their unbiased characterization for subsequent downstream applications.

