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Whole genome sequence analysis of Shigella from Malawi identifies fluoroquinolone resistance
George E Stenhouse1, Khuzwayo C Jere1,2,3, Chikondi Peno2
1University of Liverpool, Liverpool, UK.
Abstract:
Increasing antimicrobial resistance and limited alternative treatments have led to fluoroquinolone-resistant Shigella strain inclusion on the WHO global priority pathogens list. In this study we characterized multiple Shigella isolates from Malawi with whole genome sequence analysis, identifying the acquirable fluoroquinolone resistance determinant qnrS1.
Insights
Fluoroquinolone-resistant Shigella strains are a global health threat. Researchers identified the qnrS1 gene, a key factor in fluoroquinolone resistance, in Shigella isolates from Malawi.
Area of Science:
- Microbiology
- Genetics
- Public Health
Background:
- Antimicrobial resistance is a growing global health concern, necessitating research into resistant bacterial strains.
- The World Health Organization has listed fluoroquinolone-resistant Shigella as a priority pathogen due to limited treatment options.
Purpose of the Study:
- To characterize Shigella isolates from Malawi using whole genome sequencing.
- To identify specific genetic determinants of fluoroquinolone resistance in these isolates.
Main Methods:
- Whole genome sequencing of multiple Shigella isolates from Malawi.
- Bioinformatic analysis to identify resistance genes.
Main Results:
- Identification of the qnrS1 gene, an acquired fluoroquinolone resistance determinant, in the studied Shigella isolates.
- Genomic characterization of Shigella strains from Malawi.
Conclusions:
- The presence of the qnrS1 gene contributes to fluoroquinolone resistance in Shigella strains from Malawi.
- Understanding resistance mechanisms is crucial for developing effective treatment strategies against priority pathogens.
