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Updated: Nov 6, 2025
08:53
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
Published on: May 2, 2025
613
The Contribution of Lysosomes to DNA Replication
Joanna Maria Merchut-Maya1,2, Apolinar Maya-Mendoza1
1DNA Replication and Cancer Group, Danish Cancer Society Research Center, DK-2100 Copenhagen, Denmark.
Cells
|May 5, 2021
Summary
Lysosomes regulate DNA replication by controlling nucleotide synthesis and degradation. Targeting lysosome function offers new therapeutic strategies for diseases like cancer.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Lysosomes are key organelles for cellular recycling and metabolic regulation.
- They maintain energy homeostasis and supply substrates for DNA replication.
- Replication stress from nucleotide imbalance threatens genome integrity.
Purpose of the Study:
- To review the interplay between lysosomal function and DNA replication.
- To explore the roles of nucleic acid degradation and nucleotide biosynthesis.
- To discuss therapeutic exploitation of these mechanisms.
Main Methods:
- Literature review of lysosome function in DNA replication.
- Analysis of nucleotide metabolism and lysosomal pathways.
- Examination of therapeutic targets related to lysosomes and DNA synthesis.
Main Results:
- Lysosomes, via mTORC1, are crucial for purine and pyrimidine synthesis.
- Lysosomal pathways impact DNA replication fidelity and genome stability.
- Targeting lysosomal function presents therapeutic opportunities.
Conclusions:
- Lysosomal activities are integral to DNA replication and cellular health.
- Dysregulation of lysosome-nucleotide-DNA replication links to pathologies.
- Modulating lysosome function is a promising therapeutic strategy, particularly in oncology.
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