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The Peroxiredoxin Asp f3 Acts as Redox Sensor in Aspergillus fumigatus

Jana Marie Boysen1,2, Nauman Saeed1,2, Thomas Wolf3

  • 1Junior Research Group Evolution of Microbial Interactions, Leibniz-Institute for Natural Product Research and Infection Biology-Hans Knöll Institute (HKI), Beutenbergstr. 11a, 07745 Jena, Germany.

Genes
|May 5, 2021
PubMed

Insights

The fungal protein Asp f3 is crucial for invasive aspergillosis (IA) by sensing reactive oxygen species (ROS). Without Asp f3, the fungus fails to activate key oxidative stress genes and its defense mechanisms.

Area of Science:

  • Mycology
  • Immunology
  • Molecular Biology

Background:

  • * *Aspergillus fumigatus* is a human pathogen causing invasive aspergillosis (IA) in immunocompromised individuals.
  • * Fungal redox homeostasis is challenged by reactive oxygen species (ROS) during infection and antifungal treatment.
  • * The peroxiredoxin Asp f3 is essential for IA, but its role in fungal redox homeostasis is unclear.

Purpose of the Study:

  • * To investigate the function of Asp f3 in fungal redox homeostasis during infection.
  • * To determine how Asp f3 integrates with the oxidative stress response in *Aspergillus fumigatus*.

Main Methods:

  • * Gene deletion of *asp f3* in *Aspergillus fumigatus*.
  • * Analysis of global gene transcription under oxidative stress conditions.
  • * Investigation of nuclear localization of the transcription factor Afyap1.

Main Results:

  • * Asp f3 is essential for the induction of the oxidative stress response in *Aspergillus fumigatus* hyphae.
  • * Deletion of *asp f3* prevents the activation of redox-active genes, including gliotoxin biosynthesis and the Afyap1 regulon.
  • * Asp f3 acts as an intracellular redox sensor, influencing the nuclear localization of Afyap1 during ROS exposure.

Conclusions:

  • * Asp f3 plays a critical role in sensing ROS and activating fungal defense mechanisms against oxidative stress.
  • * Asp f3 is a key component in the virulence of *Aspergillus fumigatus* by modulating its response to host immunity and antifungal drugs.
Keywords:
AfYap1Aspergillus fumigatusoxidative stressperoxiredoxin Asp f3transcriptomics

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