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Updated: Nov 6, 2025
Ex Vivo Perfusion of the Rodent Placenta
Published on: May 30, 2019
Apoptotic endocrinal toxic effects of perchlorate in human placental cells
Mona M Ali1,2, Sarah A Khater3, Amel Ahmed Fayed4
1Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Cairo University, Egypt.
Background:
Perchlorate is a strong oxidizing agent and has many adverse health effects. This study investigated the potential oxidative, apoptotic, and endocrinal toxic effects of perchlorate in human placenta-derived mesenchymal stem cells (HP-MSCs).
Methods:
HP-MSCs were treated with two doses of perchlorate (5 and 15 μg/L) for three days. The perchlorate's effects were detected by histopathological examination, aromatase/CYP19 A1 activity, reactive oxygen species production (ROS), and Caspase-3 expression.
Results:
The highest perchlorate concentration (15 μg/L) caused significant placental histopathological changes. The placental cell viability was significantly affected by a significant increase in ROS generation; caspase-3 expression, and a significant reduction of CYP 19 activity. Despite the slight induction effect of the lowest perchlorate concentration (5 μg/L) on caspase 3 expression, CYP 19 activity, and ROS generation, it did not affect placental cellular viability.
Conclusion:
This study suggested that perchlorate could modulate aromatase activity and placental cytotoxicity. The continuous monitoring of the actual perchlorate exposure is needed and could be cost-effective.
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