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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Concordance between flow cytometry CLL scores.

Marc Sorigue1, Minerva Raya1, Sara Vergara1

  • 1Hematology Laboratory, ICO-Hospital Germans Trias i Pujol. Functional Cytomics- IJC, LUMN, Universitat Autònoma de Barcelona, Badalona, Spain.

International Journal of Laboratory Hematology
|May 5, 2021
PubMed
Summary

Flow cytometry diagnostic systems for classifying leukemic lymphoproliferative disorders (LPD) show poor agreement. This impacts accurate diagnosis of chronic lymphocytic leukemia (CLL) versus non-CLL LPD.

Keywords:
CD43chronic lymphocytic leukemiaflow cytometryflow cytometry scorelymphoproliferative disorder

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Area of Science:

  • Hematology
  • Immunophenotyping
  • Diagnostic Accuracy

Background:

  • Numerous flow cytometry scoring systems exist for lymphoproliferative disorders (LPD).
  • Limited comparative studies exist for these diagnostic systems.

Purpose of the Study:

  • To compare the classification agreement of eleven published flow cytometry scores for LPD.
  • To assess diagnostic concordance for chronic lymphocytic leukemia (CLL) versus non-CLL LPD.

Main Methods:

  • A cohort of leukemic LPD patients was classified using eleven distinct flow cytometry scoring systems.
  • Classification outcomes were compared to determine agreement on CLL versus non-CLL LPD.

Main Results:

  • Patient classification as CLL varied significantly (46%-73%) across systems.
  • Full agreement among all eleven systems was observed in only 57% of cases.
  • Pairwise agreement varied, with Cohen's kappa ranging from 0.48 to 0.87.

Conclusions:

  • Published flow cytometry scores/diagnostic systems demonstrate suboptimal agreement for LPD classification.
  • This variability may affect diagnostic consistency and patient management.