Related Experiment Video

Updated: Nov 6, 2025

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
08:28

Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Published on: June 30, 2017

9.6K

POU2AF1 promotes MSCs adipogenesis by inhibiting HDAC1 expression

Yaqing Wang1, Luyang Wang2, Zhongping Su3

  • 1Department of Cardiology, Geriatric Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.

Adipocyte
|May 5, 2021
PubMed

Insights

POU class 2 homeobox associating factor 1 (POU2AF1) promotes mesenchymal stem cell (MSC) adipogenesis via histone deacetylases 1 (HDAC1) signaling. Modulating POU2AF1 effectively controls adipose production, offering therapeutic potential for metabolic diseases.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Excess visceral adipose tissue is a significant risk factor for numerous diseases.
  • Inhibiting adipogenesis in mesenchymal stem cells (MSCs) is a key strategy to reduce adipose tissue accumulation.
  • The role of POU class 2 homeobox associating factor 1 (POU2AF1) in MSC adipogenesis requires further elucidation.

Purpose of the Study:

  • To investigate the role of POU2AF1 in the adipogenesis of human adipose-derived MSCs.
  • To explore the underlying signaling pathway involving histone deacetylases 1 (HDAC1).
  • To assess the potential of POU2AF1 as a therapeutic target for managing obesity and related diseases.

Main Methods:

  • Isolation of human retroperitoneal adipose-derived MSCs from overweight and control patients.
  • Gene expression analysis using IncRNA microarray.
  • Manipulation of POU2AF1 and HDAC1 levels via adenovirus transduction and small-interfering RNA.
  • Assessment of adipogenesis using Oil-red O staining, triglyceride and cholesterol assays, real-time PCR, and Western Blot.

Main Results:

  • POU2AF1 expression was elevated in overweight patients' adipose tissue and increased during adipogenesis.
  • POU2AF1 overexpression promoted MSC adipogenesis, while its silencing inhibited it.
  • POU2AF1 overexpression reduced nuclear translocation and mRNA levels of HDAC1.
  • Co-transfection with HDAC1 partially reversed POU2AF1's pro-adipogenic effects.

Conclusions:

  • POU2AF1 plays a crucial role in human MSC adipogenesis through HDAC1 signaling.
  • Modulating POU2AF1 expression offers a potential strategy to control adipose tissue development.
  • Targeting POU2AF1 may provide a novel therapeutic approach for metabolic disorders associated with excessive adipose tissue.
Keywords:
POU2AF1adipogenesishdac1mesenchymal stem cellsoverweight

More Related Videos

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

15.4K
Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System09:52

Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System

Published on: January 6, 2023

2.5K

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
4.4K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.8K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.9K