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Updated: Nov 6, 2025

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
Published on: November 23, 2015
AES and ToF-SIMS combination for single cell chemical imaging of gold nanoparticle-labeled Escherichia coli
Cécile Courrèges1, Mélanie Bonnecaze1, Delphine Flahaut1
1Université de Pau et des Pays de l'Adour, E2S UPPA, CNRS, Institut des Sciences Analytiques et Physico-Chimie Pour l'Environnement et les matériaux (IPREM), 2 avenue du Président Angot, 64000 Pau, France.
Abstract:
A chemical fingerprint of the Escherichia coli cell surface labeled by gelatin coated gold nanoparticles was obtained by combining Auger Electron Spectroscopy (AES) for single cell level chemical images, and Time-of-Flight Secondary Ion Mass Spectroscopy (ToF-SIMS) Tandem MS for unambiguous molecular identification of co-localized species.
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