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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

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Published on: June 1, 2011

Extraction and electrochemical detection for quantification of trace-level DNA.

Suparat Cotchim^1,2,3, Panote Thavarungkul^1,2,3, Proespichaya Kanatharana^1,2,3

¹Center of Excellence for Trace Analysis and Biosensor, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.

Mikrochimica Acta

|May 5, 2021

Summary

A new method efficiently extracts, detects, and quantifies trace DNA using methylene blue (MB) and magnetic nanoparticles. This DNA sensor achieves a low limit of detection, proving effective in real-world samples.

Keywords:

3-aminopropyltriethoxysilane-modified electrode Differential pulse voltammetry Electrochemical sensor Iron oxide magnetic nanoparticles Methylene blue Trace DNA

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Area of Science:

Analytical Chemistry
Biotechnology
Materials Science

Background:

Accurate detection and quantification of trace DNA are crucial in various scientific fields.
Existing methods for DNA analysis can be complex, time-consuming, or require specialized equipment.
Development of sensitive and rapid DNA sensing platforms is an ongoing research area.

Purpose of the Study:

To develop a novel, efficient strategy for the extraction, detection, and quantification of trace-level DNA.
To create a DNA sensor based on functionalized magnetic nanoparticles and electrochemical detection.
To evaluate the performance of the proposed sensor in terms of sensitivity, linearity, and accuracy in real samples.

Main Methods:

DNA extraction using a composite of methylene blue (MB), poly(acrylic acid) (PAA), and modified iron oxide magnetic nanoparticles (PAA/IOMNPs).
Separation of DNA-adsorbed nanoparticles using an external magnet and elution of MB-DNA with acetic acid.
Electrochemical detection and quantification of DNA via differential pulse voltammetry (DPV) of MB on an APTES-modified glassy carbon electrode.

Main Results:

The DNA sensor demonstrated excellent sensitivity with a limit of detection of 0.87 fg μL⁻¹.
Linear quantification ranges were observed from 0.001 to 0.400 pg μL⁻¹ under optimal conditions.
The sensor successfully detected trace DNA in real samples with high recovery rates (80.4% to 90.4%).

Conclusions:

The developed MB-PAA/IOMNPs composite and electrochemical sensor provide a sensitive and reliable method for trace DNA analysis.
This approach offers a promising alternative for rapid and efficient DNA quantification in complex matrices.
The strategy highlights the potential of magnetic nanoparticles and electrochemical techniques in advanced biosensing applications.