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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
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TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
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Tandem Mass Tags for Comparative and Discovery Proteomics.

Oliver Pagel1, Laxmikanth Kollipara1, Albert Sickmann2,3,4

  • 1Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., Bunsen-Kirchhoff-Straße 11, Dortmund, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|May 5, 2021
PubMed
Summary

This study presents a simple protocol for deep proteome quantitative analysis using Tandem Mass Tags 10plex (TMT10plex). This method enables robust relative protein quantification across multiple biological samples for discovery proteomics.

Keywords:
LC–MS/MSMultiplexingRelative quantitative proteomicsTMT

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Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Biochemistry

Background:

  • Comparative proteomics reveals altered protein abundances using LC-MS.
  • Isobaric mass tags enable simultaneous proteome identification and quantification.
  • Tandem Mass Tags (TMT) are an established technology for relative peptide/protein quantification via reporter ion intensities.

Purpose of the Study:

  • To describe a straightforward protocol for deep proteome quantitative analysis.
  • To demonstrate the utility of TMT10plex for comparative proteomics.

Main Methods:

  • Utilized Tandem Mass Tags 10plex (TMT10plex) labeling.
  • Performed liquid chromatography-mass spectrometry (LC-MS) measurements.
  • Applied a differential labeling approach for relative quantification.

Main Results:

  • The TMT10plex technology allows for multiplexing up to 10 samples.
  • The described protocol facilitates relatively deep proteome profiling.
  • Reporter ion intensities were compared for peptide/protein quantification.

Conclusions:

  • The TMT10plex protocol is effective for deep proteome quantitative analyses.
  • This method supports comparative proteomics in biological research.
  • The protocol offers a streamlined approach for TMT-based quantitative proteomics.