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Identification of the 80-kDa protein that crosslinks to the cap structure of eukaryotic mRNAs as initiation factor
S C Milburn1, J Pelletier, N Sonenberg
1Department of Biological Chemistry, School of Medicine, University of California, Davis 95616.
Archives of Biochemistry and Biophysics
|July 1, 1988
Abstract:
Preparations of either crude or purified protein synthesis initiation factors, when tested by crosslinking to the m7G-cap structure of mRNAs, exhibit specific crosslinking to an 80-kDa protein. Polyclonal antibodies specific for eIF-4B precipitate the 80-kDa cap-radiolabeled protein, thereby demonstrating that eIF-4B binds mRNA near its 5'-terminus.