Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

5.1K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.1K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.2K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.2K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

3.1K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
3.1K
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

4.7K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
4.7K
Cell-surface Signaling01:21

Cell-surface Signaling

53.0K
Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects.
53.0K
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

5.9K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chitosan-Silk Fibroin Hydrogel Scaffold Incorporating Bioactive <i>Aloe vera</i> and Mimosa Complex for Cartilage-Supportive Applications.

Polymers·2026
Same author

Development of a simple labeling method using fluorescent protein fusion proteins targeting the membrane lipids of small extracellular vesicles.

Journal of pharmaceutical and biomedical analysis·2026
Same author

Application of Droplet-Array Sandwiching Technology to Click Reactions for High-Throughput Screening.

Micromachines·2025
Same author

Enhancing Antigen Recognition of Living Cells by Site-Specific VHH Antibody Conjugation.

Chembiochem : a European journal of chemical biology·2025
Same author

Improvement of Drug Release from an Aptamer Drug Conjugate Using Reductive-sensitive Linkers for Tumor-targeted Drug Delivery.

The AAPS journal·2025
Same author

Protective role of hepatic non-parenchymal cells against drug-induced hepatocyte toxicity using perfluoropolyether-based microfluidic devices.

Toxicology letters·2025
Same journal

[Effect of Application Device Design on Scalp Delivery of Topical Minoxidil Formulations].

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026
Same journal

[Survey of Pharmaceutical Interventions in Outpatient Cancer Chemotherapy].

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026
Same journal

Effects of Fluctuating Triglyceride Levels on Initial and Steady-state Serum Amiodarone Concentrations in Japanese Patients: A Retrospective Study.

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026
Same journal

[Dynamic Oligomerization Processes and Functional Characterization of Bacterial ClpP Protease].

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026
Same journal

[Structural Analysis Using Cryo-electron Microscopy for Protein-based Drug Design].

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026
Same journal

[Expansion of Targeted Protein Degradation for Targeting Aggregate-prone Proteins and Mitochondrial Proteins].

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026
See all related articles

Related Experiment Video

Updated: Nov 6, 2025

Silicon Microchips for Manipulating Cell-cell Interaction
23:21

Silicon Microchips for Manipulating Cell-cell Interaction

Published on: August 30, 2007

10.9K

[Controlling Cell Dynamics by Cell-surface Modification].

Yuriko Higuchi1, Yoshimasa Takafuji2

  • 1Graduate School of Pharmaceutical Sciences, Kyoto University.

Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|May 6, 2021
PubMed
Summary
This summary is machine-generated.

Cell surface engineering enhances mesenchymal stem cells (MSCs) for targeted delivery. Modifying MSCs with PEG-lipid conjugates improves their adhesion to inflamed tissues, advancing cell-based therapies.

Keywords:
cell adhesioncell-based medicinedrug delivery systemmesenchymal stem cell

More Related Videos

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading
10:54

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading

Published on: May 22, 2021

5.6K
Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
12:52

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

10.2K

Related Experiment Videos

Last Updated: Nov 6, 2025

Silicon Microchips for Manipulating Cell-cell Interaction
23:21

Silicon Microchips for Manipulating Cell-cell Interaction

Published on: August 30, 2007

10.9K
Quantitative Analysis of Cell Edge Dynamics during Cell Spreading
10:54

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading

Published on: May 22, 2021

5.6K
Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
12:52

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

10.2K

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Regenerative Medicine

Background:

  • Cell surface modification is crucial for controlling cell behavior in vivo, including targeted delivery and immune evasion.
  • Conventional methods utilize functional groups for covalent modification, while noncovalent strategies like electrostatic and hydrophobic interactions are also employed.
  • Mesenchymal stem cells (MSCs) are clinically relevant for cell-based therapies, necessitating improved delivery strategies.

Purpose of the Study:

  • To investigate cell surface modification techniques for enhancing cell-based therapies.
  • To optimize PEG-lipid modifications on mesenchymal stem cells (MSCs) using cyclodextrin.
  • To evaluate the efficacy of targeted ligand-decorated MSCs for improved cell adhesion.

Main Methods:

  • Analyzed factors influencing cell surface modification using PEG-lipid, with optimization via cyclodextrin.
  • Decorated MSCs with PEG-lipid conjugates functionalized with targeting ligands (peptide or scFv) recognizing ICAM1.
  • Assessed the enhanced cell adhesion of modified MSCs on cytokine-treated endothelial cells.

Main Results:

  • Optimized PEG-lipid modification of cell surfaces was achieved using cyclodextrin.
  • Targeted ligand decoration (peptide/scFv) on MSCs significantly enhanced their adhesion to endothelial cells.
  • Demonstrated improved cell adhesion on inflamed endothelial cells, indicating potential for targeted delivery.

Conclusions:

  • Cell surface engineering offers a powerful approach to improve cell-based therapies.
  • Targeted MSC delivery can be achieved through surface functionalization with specific ligands.
  • This technique holds promise for directing MSCs to specific therapeutic sites, enhancing treatment efficacy.