Related Experiment Video
Updated: Nov 6, 2025
10:28
A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
Published on: June 26, 2020
10.0K
Isolation and Characterization of Hepatitis E Virus Subtype 4b Using a PLC/PRF/5 Cell-Derived Cell Line Resistant to
Wenjing Zhang1, Michiyo Kataoka2, Hai Yen Doan3
1Department of Virology II, National Institute of Infectious Diseases, Japan.
Japanese Journal of Infectious Diseases
|May 6, 2021
Summary
A new cell line, N1380, derived from PLC/PRF/5 cells, enables successful isolation of hepatitis E virus (HEV) from swine feces. This overcomes challenges posed by porcine sapelovirus (PSV) co-infections, facilitating HEV research.
Area of Science:
- Virology
- Cell Biology
Background:
- The PLC/PRF/5 cell line is susceptible to hepatitis E virus (HEV) and used for its isolation.
- Porcine sapelovirus (PSV) co-infection in PLC/PRF/5 cells causes cytopathic effects that inhibit HEV growth, complicating isolation from animal samples.
Purpose of the Study:
- To develop a cell line resistant to PSV for improved HEV isolation from swine and wild boar fecal specimens.
- To demonstrate the utility of the N1380 cell line for isolating HEV in the presence of PSV.
Main Methods:
- Derivation of a PSV-resistant cell line (N1380) from PLC/PRF/5 cells.
- Isolation of a HEV-4b strain from a PSV-positive swine fecal specimen using the N1380 cell line.
Main Results:
- The N1380 cell line demonstrated resistance to PSV infection.
- Successful isolation of a HEV-4b strain from a mixed-infected fecal specimen was achieved using N1380 cells.
Conclusions:
- The N1380 cell line is a valuable tool for HEV isolation from swine and wild boar, even when PSV is present.
- This advancement facilitates the study and surveillance of HEV in animal populations.
