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miR-139-5p Regulates the Proliferation of Acute Promyelocytic Leukemia Cells by Targeting MNT

Yueyue Fu1, Limin Li2, Jinxiao Hou1

  • 1Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, Heilongjiang, China.

Journal of Oncology
|May 6, 2021
PubMed

Insights

MicroRNAs regulate white blood cell counts in acute promyelocytic leukemia (APL). Downregulated miR-139-5p is linked to leukocytosis, and restoring its levels may offer new APL treatment strategies.

Area of Science:

  • Hematology
  • Molecular Biology
  • Oncology

Background:

  • Progressive leukocytosis in acute promyelocytic leukemia (APL) correlates with poor outcomes.
  • The specific microRNA (miRNA) roles in APL leukocytosis remain unclear.

Purpose of the Study:

  • To investigate the impact of miRNAs on leukocytosis during APL induction therapy.
  • To explore the underlying mechanisms of miRNA regulation in APL.

Main Methods:

  • Patients undergoing induction therapy were categorized into leukocytosis and non-leukocytosis groups based on white blood cell counts.
  • Microarray analysis was employed to identify differentially expressed miRNAs.
  • In vitro studies using NB4 cells assessed the functional effects of miR-139-5p and its target MNT.

Main Results:

  • miR-139-5p was significantly downregulated in APL patients with leukocytosis.
  • Increased miR-139-5p expression suppressed NB4 cell proliferation via cell cycle arrest and apoptosis induction.
  • MNT was identified as a direct target of miR-139-5p, mediating its effects on cell proliferation, invasion, and migration.

Conclusions:

  • miR-139-5p plays a crucial role in controlling leukocytosis in APL.
  • Targeting miR-139-5p or its downstream effector MNT presents a potential therapeutic avenue for managing progressive leukocytosis in APL.

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