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Updated: Jul 12, 2026

Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field
Published on: August 2, 2011
Detection and molecular characterization of infectious bronchitis virus from recent outbreaks in broiler flocks in
Hana Sherzad Raoof1, Mohammed Omar Baba Sheikh2, Rizgar Raheem Sulaiman1
1Department of Clinic and Internal Medicine, College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, Iraq.
Abstract:
Infectious Bronchitis (IB) is one of the most important viral diseases of poultry. It causes major financial losses in the poultry industry. It is caused by the infectious bronchitis virus (IBV). This study investigated the genetic origin and diversity of IBV by analyzing the partial S1 sequence derived from local broiler farms in different regions of Sulaimani Governorate. Detection was performed using a newly designed primer for the partial S1 gene. The present was undertaken to understand the molecular relationship between the strains across the world compared with field virus isolates by phylogenetic tree and sequences-analysis. Phylogenetic tree demonstrated that field isolate made a novel group (sul-5/17-like strain), indicating new variants. Also, field virus sequences were closely matched to Iranian strain HQ123347/RFIBV6 (97.00%). Further sequences analysis exhibited that field isolate was not homologous but high divergences with reverence vaccines strains. These results indicated that differences in S1 protein of the locally circulated IBV could be a reason for currents vaccination failure.
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