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Updated: Nov 6, 2025
Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
Mass Spectrometric Analysis of Bioactive Sphingolipids in Fungi
Ashutosh Singh1, Maurizio Del Poeta2,3,4
1Department of Biochemistry, University of Lucknow, Lucknow, Uttar Pradesh, India. singh_ashutosh@lkouniv.ac.in.
Abstract:
As biomolecules, sphingolipids represent a broad spectrum of structures ranging from simple long chain bases to complex glycosphingolipids. While several different mass spectrometry based approaches have been proven to be useful in qualitative and quantitative analysis of sphingolipids, we find that electrospray ionization tandem mass spectrometry (ESI-MS/MS) in the multiple-reaction monitoring (MRM) mode using a triple quadrupole instrument, coupled to high-performance liquid chromatography (HPLC), is the most suitable approach for the analysis. In this chapter, we describe the method in a step-by-step manner towards the targeted analysis of sphingolipids in fungi. With optimized HPLC separation and instrument settings, this MRM approach affords detection of many sphingolipid species simultaneously with good sensitivity.
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