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Related Experiment Video

Updated: Nov 6, 2025

Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood

Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood

Published on: February 5, 2020

PD-1 blocking antibodies moonlighting as killers.

Judith Leitner¹, Claire Battin¹, Katharina Grabmeier-Pfistershammer¹

¹Division of Immune Receptor and T-Cell Activation, Institute of Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.

European Journal of Immunology

|May 6, 2021

Summary

Blocking PD-1 antibodies in cancer research can deplete T cells, impacting immunotherapy study outcomes. Thorough antibody characterization is crucial for accurate preclinical immunotherapy results.

Keywords:

ADCC Immune checkpoint inhibitors Immunotherapy PD-1 antibodies

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Published on: July 7, 2023

Area of Science:

Immunology
Cancer Therapy
Preclinical Research

Background:

Therapeutic antibodies blocking PD-1 have transformed cancer treatment.
Murine cancer models are vital for evaluating PD-1 inhibitors.
Antibody isotype and host species can influence blocking antibody efficacy and potential cytotoxicity.

Purpose of the Study:

To investigate the impact of PD-1 blocking antibodies on T cell populations in preclinical immunotherapy models.
To highlight the importance of comprehensive antibody characterization in immunotherapy research.

Main Methods:

Administration of PD-1 blocking antibodies in different in vivo murine models.
Analysis of T cell populations, including antigen-specific CD8 T cells.
Evaluation of antibody-mediated effects on T cell activation, proliferation, and survival.

Main Results:

Certain PD-1 antibodies promoted activation and proliferation of PD-1+ T cells.
A specific PD-1 antibody led to significant depletion of antigen-specific CD8 T cells in vivo.
The observed T cell depletion varied depending on the antibody used and experimental model.

Conclusions:

"Blocking" PD-1 antibodies can induce unintended T cell depletion, affecting immunotherapy study outcomes.
Comprehensive characterization of PD-1 antibodies is essential to prevent misinterpretation of preclinical immunotherapy data.
Understanding antibody-mediated effector functions is critical for reliable cancer immunotherapy research.