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Plasma creatine determination using a luminescence method.

C M Jabs1, P Nëglen, B Eklöf

  • 1Department of Surgery, Kuwait University, Safat.

Biochemical Medicine and Metabolic Biology
|June 1, 1988
PubMed
Summary
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A new luminescence assay accurately measures plasma creatine using only 20 microliters of sample. This method is ideal for small animal studies and shows potential for early detection of cellular damage.

Area of Science:

  • Biochemistry
  • Clinical Chemistry

Background:

  • Plasma creatine measurement is crucial for diagnosing cellular damage.
  • Existing methods often require large sample volumes, limiting their use in small animal research.

Purpose of the Study:

  • To develop a novel, sensitive luminescence-based assay for plasma creatine.
  • To validate the assay's performance and assess its applicability in clinical and small animal settings.

Main Methods:

  • A luminescence procedure utilizing the creatine kinase reaction was established.
  • Plasma samples were deproteinized using ethanol at room temperature.
  • Assay performance was evaluated for sample volume, stability, precision, and correlation with the NADH spectrophotometric method.

Main Results:

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  • The luminescence assay requires only 20 microliters of plasma.
  • Creatine levels remained stable in plasma for at least 1 hour with 10 mM EDTA.
  • Excellent correlation (r = 0.99) was observed with the NADH spectrophotometric method, with high within-run precision (2-3% CV).
  • Preliminary studies indicated significantly elevated plasma creatine levels in conditions of muscle ischemia and shock.

Conclusions:

  • The developed luminescence assay is a sensitive, accurate, and efficient method for plasma creatine determination.
  • Its low sample volume requirement makes it highly suitable for small animal research and clinical applications.
  • Elevated plasma creatine may serve as an early indicator of cellular damage with potential prognostic value.