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MicroRNA-543-3p down-regulates inflammation and inhibits periodontitis through KLF6

Wei Li1, Junwei Wang2, Wenjing Hao1

  • 1Department of Stomatology, The Affiliated Hospital of Qingdao University, No. 16 Jiangsu Road, Shinan District, Qingdao 266000, Shandong, China.

Bioscience Reports
|May 6, 2021
PubMed

Insights

MicroRNA-543-3p is down-regulated in periodontitis, promoting inflammation and cell death. Increasing miR-543-3p levels may combat inflammation and inhibit periodontitis by targeting KLF6.

Area of Science:

  • Molecular Biology
  • Immunology
  • Periodontology

Background:

  • MicroRNA-543-3p (miR-543-3p) involvement in human diseases is known, but its role in inflammation remains unclear.
  • Bacterial infections trigger inflammation via lipopolysaccharide (LPS) activating innate immune cells.

Purpose of the Study:

  • To investigate the role of miR-543-3p in lipopolysaccharide (LPS)-induced inflammation in periodontal ligament cells (PDLCs).
  • To elucidate the molecular mechanism of miR-543-3p in periodontitis development.

Main Methods:

  • Down-regulation of miR-543-3p was observed in LPS-treated PDLCs.
  • The effect of miR-543-3p on LPS-induced apoptosis and inflammation in PDLCs was assessed.
  • Kruppel-like factor 6 (KLF6) was identified as a direct downstream target of miR-543-3p.

Main Results:

  • miR-543-3p was down-regulated in LPS-treated PDLCs.
  • miR-543-3p mediated LPS-induced PDLC apoptosis and inflammatory damage.
  • Up-regulation of miR-543-3p alleviated LPS-induced inflammatory damage.
  • KLF6 was confirmed as a direct target of miR-543-3p, playing a role in periodontitis.

Conclusions:

  • miR-543-3p plays a protective role against inflammation and apoptosis in PDLCs.
  • miR-543-3p inhibits periodontitis progression by down-regulating inflammation via targeting KLF6.
  • miR-543-3p represents a potential therapeutic target and biomarker for periodontitis.
Keywords:
Kruppel-like factor 6LPS: lipopolysaccharidenormal control

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