A Novel Method to Gently Mix and Uniformly Suspend Particulates for Automated Assays

Area of Science:

• Biotechnology
• Laboratory Automation
• Assay Development

Background:

• Automated assays require uniform suspension of particulates like cells and beads.
• Traditional mixing methods can compromise cell viability and bead morphology.
• Efficient and gentle mixing is crucial for reliable assay results.

Purpose of the Study:

• To introduce and evaluate the SpinVessel system for particulate suspension in laboratory automation.
• To demonstrate the system's ability to maintain particulate integrity during mixing.
• To assess the uniformity and consistency of aliquots generated using the SpinVessel.

Main Methods:

• The SpinVessel system utilizes internal fins and rapid spin reversals to create pulsed radial flow.
• Five different particulates (cells, magnetic beads, silica beads, microcarrier beads) were tested.
• Six SpinVessel sizes (50 mL to 1200 mL) were evaluated for compatibility with various pipetting systems.

Main Results:

• High cell viability and undamaged microcarrier bead morphology were observed after hours of agitation.
• Uniform suspension of particulates was achieved throughout the vessel's height and breadth.
• Consistent aliquot uniformity was maintained throughout the aliquoting process.
• Settled particulates were resuspended in only 5 minutes.

Conclusions:

• The SpinVessel system offers a novel and effective method for gentle, uniform particulate suspension.
• It is compatible with standard laboratory automation equipment and various particulate types.
• This technology is highly applicable to automated assays demanding particulate uniformity and integrity.