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[Extracellular ribonuclease from Bacillus thuringiensis]
Biokhimiia (Moscow, Russia)
|April 1, 1988
Summary
Bacillus thuringiensis var. subtoxicus produces extracellular ribonuclease (ribonuclease Bt) with RNA-depolymerizing activity. This enzyme, purified via chromatography, functions optimally at pH 8.5 and exhibits guanylic specificity.
Area of Science:
- Microbiology
- Enzymology
- Biochemistry
Context:
- Investigating microbial enzyme production in Bacillus strains.
- Characterizing extracellular enzymes involved in RNA degradation.
- Exploring the enzymatic properties of Bacillus thuringiensis var. subtoxicus.
Purpose:
- To study the production and properties of extracellular ribonuclease (ribonuclease Bt) from Bacillus thuringiensis var. subtoxicus.
- To purify and characterize the ribonuclease enzyme.
- To determine the optimal conditions and substrate specificity of the enzyme.
Summary:
- Bacillus thuringiensis var. subtoxicus secretes an extracellular ribonuclease (ribonuclease Bt) with peak activity 4-5 hours post-growth.
- A homogeneous enzyme (12000 Da) was isolated using three-step phosphocellulose chromatography.
- The enzyme exhibits maximum RNA-depolymerizing activity at pH 8.5, hydrolyzing polyribonucleotides and guanosine-2',3'-cyclophosphate.
Impact:
- Provides insights into the enzymatic capabilities of Bacillus thuringiensis strains.
- Characterization of a novel ribonuclease with potential applications in biotechnology.
- Contributes to the understanding of microbial RNA-processing enzymes.