[Effect of cycloheximide on transcription in eukaryotic cells]

The effects of the translation inhibitor cycloheximide on transcription in Rauscher virus transformed mouse erythroblasts and in barley rootlets were studied. In transformed erythroblasts, the globin gene transcription appeared to be blocked. Consecutive treatment of cells with sublethal doses of cycloheximide (7 micrograms/ml) and actinomycin D resulted in the induction of synthesis of the protein fraction corresponding to beta-globin chains. An exposure of barley rootlets to cycloheximide caused changes in total RNA synthesis.