Secure and optimized detection of PNPLA3 rs738409 genotype by an improved PCR-restriction fragment length
Jörg Enssle1,2, Karsten-Henrich Weylandt1,2,3
1Faculty of Health Sciences, Joint Faculty of The Brandenburg University of Technology Cottbus-Senftenberg, The Brandenburg Medical School Theodor Fontane & The University of Potsdam, Karl-Liebknecht-Straße 24-25, Potsdam, 14476, Germany.
Abstract:
The PNPLA3 reference single-nucleotide polymorphism rs738409 has been identified as a predisposing factor for nonalcoholic fatty liver disease. A simple method based on PCR and restriction fragment length polymorphism (RFLP) analysis had been published to detect the nonpathogenic allele PNPLA3 rs738409 variant. The presence of the pathogenic variant was deduced by the indigestibility of the corresponding PCR product with BtsCI recognizing the nonpathogenic allele. However, one cannot exclude that an enzymatic reaction does not occur for other, more trivial, reasons. For safe and secure detection of the pathogenic PNPLA3 rs738409, we have further developed the PCR-restriction fragment length polymorphism method by adding a second restriction enzyme digest, clearly identifying the correct PNPLA3 alleles and in particular the pathogenic variant.
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