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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Abnormal Proliferation02:23

Abnormal Proliferation

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Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
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lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Receptor Downregulation in MVBs01:15

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Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
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Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
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Related Experiment Video

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LINC00908 Promotes Diffuse Large B-Cell Lymphoma Development by Down-Regulating miR-671-5p.

Hong Zeng1, Yongqiang Wei1, Xiaolei Wei1

  • 1Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.

Cancer Management and Research
|May 7, 2021
PubMed
Summary

Long noncoding RNA LINC00908 acts as an oncogene in diffuse large B-cell lymphoma (DLBCL). It promotes DLBCL malignancy by regulating microRNA miR-671-5p, inhibiting cancer cell proliferation and invasion.

Keywords:
LINC00908diffuse large B-cell lymphomainvasionmiR-671-5pproliferation

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An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in human cancers.
  • LINC00908 is an oncogene in prostate, colorectal, and gastric cancers.
  • The role of LINC00908 in diffuse large B-cell lymphoma (DLBCL) is not well understood.

Purpose of the Study:

  • To investigate the biological role and molecular mechanism of LINC00908 in DLBCL.
  • To determine if LINC00908 functions as an oncogene in DLBCL.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) to assess LINC00908 and miR-671-5p expression in DLBCL tissues and cell lines.
  • Cell proliferation (CCK-8) and invasion (Transwell) assays to evaluate LINC00908's in vitro role.
  • Xenograft models for in vivo assessment of LINC00908's role in DLBCL growth.
  • Bioinformatics, dual-luciferase assays, RNA immunoprecipitation (RIP), and RNA pull-down assays to confirm the interaction between LINC00908 and miR-671-5p.

Main Results:

  • LINC00908 expression was significantly upregulated in DLBCL tissues and cell lines.
  • Decreased LINC00908 expression inhibited DLBCL cell proliferation and invasion.
  • LINC00908 directly targets and downregulates miR-671-5p, which is downregulated in DLBCL.
  • LINC00908 acts as an oncogene in DLBCL by regulating miR-671-5p, and its silence inhibited DLBCL growth in vivo.

Conclusions:

  • LINC00908 promotes the malignancy of diffuse large B-cell lymphoma.
  • This oncogenic role is mediated through the regulation of miR-671-5p.