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Updated: Nov 6, 2025

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
An electronic circular dichroism spectroscopy method for the quantification of L- and D-amino acids in enantiomeric
Ruiwen Ding1,2, Jianxi Ying1,2, Yufen Zhao1,2
1Institute of Drug Discovery Technology, Ningbo University, No. 818 Fenghua Road, Ningbo, Zhejiang 315211, People's Republic of China.
Abstract:
Rapid quantitative analysis of single chiral amino acid (aa) was achieved using circular dichroism (CD) with data analysis by standard calibration curve. The absolute concentrations of D- and L-aas in enantiomeric mixtures were determined by CD and achiral liquid chromatography (LC) method. It is worth noting that CD and LC were used independently, not online LC/CD in this study. The errors of the experimental results were less than 10%. The method is also applicable to the quantification of non-aa chiral molecules, such as chiral nucleoside and chiral quinine. With this study, we provide a new method for the chiral quantitative analysis of enantiomeric aas mixtures.
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