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Antigenotoxic potential of the fermentation broth produced by Paenibacillus polymyxa RNC-D in vitro
Jaqueline Bianchi1, Rafael Cavicchioli1, Lauro T Kubota2,3
1Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos (UFSCar), São Carlos, 13565-905, SP, Brazil.
Abstract:
Aim: Evaluate the chemopreventive potential of the extract from P. polymyxa RNC-D. Methods: Concentrations of P. polymyxa RNC-D extract were tested in HepG2/C3A cells to assess their genotoxic (comet assay), mutagenic (micronucleus test) and antigenotoxic potential (comet assay) in vitro. Results: 400 and 40 μg/ml concentrations induced DNA lesions, whereas the 4 μg/ml induced a desmutagenic effect. Complementary tests indicated that the extract minimized the formation of reactive oxygen species induced by methyl methanesulfonate and normalized the loss of membrane potential. The quantification of cytokines indicated that TNF-α was immunostimulated by the extract. However, when administered in conjunction with the methyl methanesulfonate, the extract blocked the TNF-α release. Conclusion: The fermentation broth from P. polymyxa RNC-D showed an antigenotoxic effect, and thus the potential to be used as chemopreventive compound.
Insights
The *P. polymyxa* RNC-D extract demonstrated antigenotoxic effects, suggesting its potential as a chemopreventive compound. It protected against DNA damage and reduced harmful reactive oxygen species formation.
Area of Science:
- Microbiology
- Genetics
- Toxicology
Background:
- Chemoprevention strategies are crucial for mitigating cancer risk.
- Natural product extracts are increasingly investigated for therapeutic properties.
- Understanding the genotoxic and antigenotoxic effects of microbial metabolites is essential.
Purpose of the Study:
- To evaluate the chemopreventive potential of *P. polymyxa* RNC-D extract.
- To assess the genotoxic, mutagenic, and antigenotoxic effects of the extract *in vitro*.
- To investigate the extract's impact on cellular stress markers and cytokine production.
Main Methods:
- *In vitro* testing of *P. polymyxa* RNC-D extract on HepG2/C3A cells.
- Comet assay for genotoxicity and antigenotoxicity assessment.
- Micronucleus test for mutagenicity evaluation.
- Measurement of reactive oxygen species and membrane potential.
- Quantification of Tumor Necrosis Factor-alpha (TNF-α).
Main Results:
- A concentration of 4 μg/ml exhibited a desmutagenic effect, reducing DNA damage.
- The extract minimized reactive oxygen species formation induced by methyl methanesulfonate.
- It normalized loss of membrane potential and immunostimulated TNF-α.
- The extract blocked TNF-α release when co-administered with methyl methanesulfonate.
Conclusions:
- The fermentation broth of *P. polymyxa* RNC-D possesses significant antigenotoxic properties.
- The extract shows promise as a chemopreventive agent.
- Further research is warranted to explore its therapeutic applications.
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