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Nuclear matrix-intermediate filament system and its alteration in adenovirus infected HeLa cell
1Department of Biology, Peking University Beijing, People's Republic of China.
Abstract:
The nuclear matrix-intermediate filament (NM-IF) of normal and adenovirus infected HeLa cells were investigated by means of both electron microscopy and two-dimensional gel electrophoresis. After infection there were some changes in NM-IF, and the viral factory was suspended in nuclear matrix. Two new polypeptides appeared in 2-D gel of NM-IF after infection. They are probably not viral proteins but cellular polypeptides. During viral replication, all of vimentin were degraded and phosphorylated keratin 18 increased. These facts suggest that NM-IF plays a certain role in adenovirus replication.
Insights
Nuclear matrix-intermediate filaments (NM-IF) undergo changes during adenovirus infection, with new cellular proteins appearing and vimentin degrading. These alterations suggest NM-IF
Area of Science:
- Cell Biology
- Virology
- Molecular Biology
Background:
- The nuclear matrix-intermediate filament (NM-IF) network is crucial for nuclear structure and function.
- Adenovirus infection significantly alters cellular processes, including nuclear organization.
Purpose of the Study:
- To investigate the structural and compositional changes in the nuclear matrix-intermediate filament (NM-IF) network of HeLa cells upon adenovirus infection.
- To identify potential roles of NM-IF in the viral replication cycle.
Main Methods:
- Electron microscopy was used to visualize structural alterations in the NM-IF network.
- Two-dimensional gel electrophoresis was employed to analyze protein composition changes in the NM-IF.
Main Results:
- Adenovirus infection induced observable changes in the NM-IF structure, with viral factories observed within the nuclear matrix.
- Two novel polypeptides, likely of cellular origin, were detected in the NM-IF of infected cells.
- Vimentin was degraded, while phosphorylated keratin 18 levels increased during viral replication.
Conclusions:
- The nuclear matrix-intermediate filament network undergoes significant remodeling during adenovirus infection.
- Specific changes in NM-IF composition, including vimentin degradation and keratin 18 phosphorylation, are associated with adenovirus replication.
- These findings suggest a functional role for the NM-IF network in supporting adenovirus replication.