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Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

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Related Experiment Video

Updated: Nov 6, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

PacBio-Based Protocol for Bacterial Genome Assembly.

Agata Motyka-Pomagruk¹, Sabina Zoledowska^1,2, Michal Kabza³

¹Department of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk & Medical University of Gdansk, University of Gdansk, Gdansk, Poland.

Methods in Molecular Biology (Clifton, N.J.)

|May 7, 2021

Summary

This summary is machine-generated.

This study presents an accessible bacterial genome assembly pipeline using open-source software. It enables researchers to obtain high-quality bacterial genomes, aiding in pathogen detection and identification.

Keywords:

Dickeya spp.Next generation sequencing Pectobacterium spp.Single-molecule real-time sequencing Soft rot bacteria Whole-genome sequencing

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Last Updated: Nov 6, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

Area of Science:

Microbiology
Genomics
Bioinformatics

Background:

High-quality bacterial genome acquisition is crucial for understanding intraspecies variation and developing pathogen detection tools.
Long-read sequencing technologies, like Pacific Biosciences, offer potential for comprehensive genomic analysis.

Purpose of the Study:

To describe a user-friendly bacterial genome assembly pipeline.
To facilitate the transformation of sequencing data into reliable biological information for non-bioinformaticians.

Main Methods:

Development of a bacterial genome assembly pipeline utilizing open-source software.
Application of the pipeline on a standard desktop computer.

Main Results:

Successfully closed six *Dickeya solani* bacterial genomes.
Demonstrated the pipeline's efficiency and accessibility for researchers without extensive bioinformatics expertise.

Conclusions:

The developed pipeline provides an accessible method for high-quality bacterial genome assembly.
This approach supports the investigation of bacterial intraspecies variation and the development of diagnostic tools.