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LncRNA SAMD12-AS1 Promotes the Progression of Gastric Cancer via DNMT1/p53 Axis
Guang-Hui Lu1, Hui-Meng Zhao2, Zi-Yuan Liu3
1Department of General Surgery, The No.967 Hospital of PLA Joint Logistics Support Force, Postgraduate Culture Base of Jinzhou Medical University, Dalian, China.
Archives of Medical Research
|May 8, 2021
Summary
Long noncoding RNA SAMD12-AS1 promotes gastric cancer progression by inhibiting the P53 signaling pathway. This lncRNA may serve as a novel biomarker and therapeutic target for gastric cancer.
Area of Science:
- Oncology
- Molecular Biology
- Gene Regulation
Background:
- Long noncoding RNAs (lncRNAs) are key regulators in cancer development.
- LncRNA SAMD12-AS1 is implicated in various cancers, but its role in gastric cancer (GC) is not well-defined.
Purpose of the Study:
- To investigate the function and mechanism of lncRNA SAMD12-AS1 in gastric cancer progression.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) for expression analysis.
- Cell proliferation (CCK-8), cell cycle (flow cytometry), and protein levels (Western blotting) assays.
- RNA immunoprecipitation (RIP) to identify RNA-protein interactions.
Main Results:
- SAMD12-AS1 was significantly upregulated in GC tissues and cell lines.
- High SAMD12-AS1 expression correlated with advanced TNM stage and poor prognosis.
- SAMD12-AS1 promoted GC cell proliferation and inhibited the P53 signaling pathway, potentially via interaction with DNMT1.
Conclusions:
- SAMD12-AS1 promotes gastric cancer progression through the DNMT1/P53 axis.
- SAMD12-AS1 presents potential as a novel biomarker and therapeutic target for gastric cancer.
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