Oxygen Generation via Water Splitting by a Novel Biogenic Metal Ion-Binding Compound

Methanobactins (MBs) are small (<1,300-Da) posttranslationally modified copper-binding peptides and represent the extracellular component of a copper acquisition system in some methanotrophs. Interestingly, MBs can bind a range of metal ions, with some being reduced after binding, e.g., Cu²⁺ reduced to Cu⁺. Other metal ions, however, are bound but not reduced, e.g., K⁺. The source of electrons for selective metal ion reduction has been speculated to be water but never empirically shown. Here, using H₂¹⁸O, we show that when MBs from Methylocystis sp. strain SB2 (MB-SB2) and Methylosinus trichosporium OB3b (MB-OB3) were incubated in the presence of either Au³⁺, Cu², or Ag⁺, ^18,18O₂ and free protons were released. No ^18,18O₂ production was observed in the presence of either MB-SB2 or MB-OB3b alone, gold alone, copper alone, or silver alone or when K⁺ or Mo²⁺ was incubated with MB-SB2. In contrast to MB-OB3b, MB-SB2 binds Fe³⁺ with an N₂S₂ coordination and will also reduce Fe³⁺ to Fe²⁺. Iron reduction was also found to be coupled to the oxidation of 2H₂O and the generation of O₂. MB-SB2 will also couple Hg²⁺, Ni²⁺, and Co²⁺ reduction to the oxidation of 2H₂O and the generation of O₂, but MB-OB3b will not, ostensibly as MB-OB3b binds but does not reduce these metal ions. To determine if the O₂ generated during metal ion reduction by MB could be coupled to methane oxidation, ¹³CH₄ oxidation by Methylosinus trichosporium OB3b was monitored under anoxic conditions. The results demonstrate that O₂ generation from metal ion reduction by MB-OB3b can support methane oxidation. IMPORTANCE The discovery that MB will couple the oxidation of H₂O to metal ion reduction and the release of O₂ suggests that methanotrophs expressing MB may be able to maintain their activity under hypoxic/anoxic conditions through the "self-generation" of dioxygen required for the initial oxidation of methane to methanol. Such an ability may be an important factor in enabling methanotrophs to not only colonize the oxic-anoxic interface where methane concentrations are highest but also tolerate significant temporal fluctuations of this interface. Given that genomic surveys often show evidence of aerobic methanotrophs within anoxic zones, the ability to express MB (and thereby generate dioxygen) may be an important parameter in facilitating their ability to remove methane, a potent greenhouse gas, before it enters the atmosphere.