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Updated: Nov 6, 2025

An Integrated Approach for Microprotein Identification and Sequence Analysis
Published on: July 12, 2022
BENZ WS: the Bologna ENZyme Web Server for four-level EC number annotation
Davide Baldazzi1, Castrense Savojardo1, Pier Luigi Martelli1
1Biocomputing Group, Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Abstract:
The Bologna ENZyme Web Server (BENZ WS) annotates four-level Enzyme Commission numbers (EC numbers) as defined by the International Union of Biochemistry and Molecular Biology (IUBMB). BENZ WS filters a target sequence with a combined system of Hidden Markov Models, modelling protein sequences annotated with the same molecular function, and Pfams, carrying along conserved protein domains. BENZ returns, when successful, for any enzyme target sequence an associated four-level EC number. Our system can annotate both monofunctional and polyfunctional enzymes, and it can be a valuable resource for sequence functional annotation.
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