Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimodal characterization of variation in neuronal types in the mouse basal ganglia.

bioRxiv : the preprint server for biology·2026
Same author

Connecting single-cell transcriptomes to projectomes in the mouse visual cortex.

Nature·2026
Same author

Assembly and Reactions of Artificial Metalloenzymes in <i>Streptomyces albus</i>.

Journal of the American Chemical Society·2026
Same author

Morphoelectric Diversity and Specialization of Neuronal Cell Types in the Primate Striatum.

bioRxiv : the preprint server for biology·2026
Same author

TridentSynth: a webtool for the retrosynthesis of molecules using chimeric type I polyketide synthases and chemoenzymatic pathways.

Nucleic acids research·2026
Same author

Influence of titer, rate, yield, and scale on the cost and life-cycle emissions of biomanufacturing.

Trends in biotechnology·2026

Related Experiment Video

Updated: Nov 6, 2025

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
11:31

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

Published on: June 30, 2016

8.8K

Engineering Pseudomonas putida for efficient aromatic conversion to bioproduct using high throughput screening in a

Thomas Eng1, Deepanwita Banerjee1, Andrew K Lau1

  • 1Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, 5885, Hollis Street, Emeryville, CA, USA; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, Berkeley, CA, USA.

Metabolic Engineering
|May 8, 2021
PubMed
Summary

Pseudomonas putida KT2440 biomanufacturing hosts were optimized using gene deletions. Several mutants showed enhanced fitness and improved production of indigoidine from para-coumarate.

Keywords:
BioreactorMetabolic engineeringPara-coumaratePseudomonas putidaRB-TnSeq

More Related Videos

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow
09:05

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow

Published on: October 17, 2025

157
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

9.0K

Related Experiment Videos

Last Updated: Nov 6, 2025

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
11:31

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

Published on: June 30, 2016

8.8K
Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow
09:05

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow

Published on: October 17, 2025

157
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

9.0K

Area of Science:

  • Microbial biotechnology
  • Synthetic biology
  • Metabolic engineering

Background:

  • Pseudomonas putida KT2440 is a versatile microbial host for biomanufacturing.
  • Its utilization of renewable aromatic compounds like para-coumarate is of significant interest.
  • Optimizing P. putida for industrial bioprocesses requires understanding gene essentiality and fitness.

Purpose of the Study:

  • To identify genes in P. putida KT2440 whose disruption enhances microbial fitness in bioreactor conditions.
  • To evaluate the impact of specific gene deletions on the production of the bioproduct indigoidine from para-coumarate.

Main Methods:

  • Construction and screening of a pooled transposon mutant library of P. putida KT2440.
  • Quantitative fitness assays under stirred-tank bioreactor conditions.
  • Reconstruction and characterization of selected deletion mutants for bioconversion performance.

Main Results:

  • 33 gene mutants exhibiting improved fitness in bioreactors were identified.
  • Reconstructed deletion strains, including ΔgacA, ΔttgB, and ΔPP_0063, were characterized.
  • The ΔgacA mutant demonstrated a 35-fold increase in bioreactor fitness and an 8-fold improvement in indigoidine production (4.5 g/L).

Conclusions:

  • Gene disruption strategies can significantly enhance the performance of P. putida KT2440 in biomanufacturing.
  • Specific mutants, like ΔgacA, show promise for improving the yield of valuable bioproducts from renewable feedstocks.
  • This study provides valuable genetic targets for optimizing P. putida-based bioprocesses.