Study on structural characterization, physicochemical properties and digestive properties of euryale ferox resistant...

Area of Science:

• Food Science
• Nutritional Biochemistry
• Material Science

Background:

• Industrial production of resistant starch type 3 (RS3) often uses simple raw materials with low purity.
• Limited research exists on the relationship between RS3 structure and its digestion characteristics.

Purpose of the Study:

• To prepare RS3 from Euryale Ferox using diverse methods.
• To investigate the structural and physicochemical properties of Euryale Ferox-derived RS3.
• To analyze the digestion characteristics and thermal stability of modified RS3.

Main Methods:

• Euryale Ferox starch was modified using autoclaving, enzymolysis-autoclaving, and dual enzymolysis.
• Structural analysis included X-ray diffraction (XRD) for crystal type and thermal analysis (DSC).
• In vitro digestion assays were performed to determine digestion rate and estimate glycemic index (eGI).

Main Results:

• Significant differences in structure and properties were observed among Euryale Ferox RS3 prepared by different methods (p < 0.05).
• Purified RS3 exhibited a B+V crystal type and high thermal stability.
• Resistant-enhanced RS3 showed substantial changes in structure and thermal properties post-digestion.
• All three purified RS3 types demonstrated low digestion rates (<20%) and low estimated glycemic index (eGI < 50).

Conclusions:

• Euryale Ferox is a viable source for producing RS3 with tunable structural and digestion properties.
• Modified RS3 from Euryale Ferox exhibits desirable characteristics like low digestibility and high thermal stability.
• Understanding the structure-property-digestion relationships is crucial for developing functional RS3 ingredients.