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Related Experiment Videos

Nitroalkane oxidation by streptomycetes.

M R Dhawale, U Hornemann

    Journal of Bacteriology
    |February 1, 1979
    PubMed
    Summary

    Streptomyces strains oxidize nitroalkanes, producing nitrous acid. A purified enzyme from Streptomyces achromogenes catalyzes 2-nitropropane oxidation, suggesting a role in antibiotic biosynthesis.

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    Area of Science:

    • Microbiology
    • Biochemistry

    Background:

    • Nitroalkanes are utilized by some microorganisms.
    • Streptomyces species are known for producing diverse bioactive compounds, including antibiotics.

    Purpose of the Study:

    • To investigate the nitroalkane-oxidizing activity in Streptomyces strains.
    • To partially purify and characterize a nitroalkane-oxidizing enzyme from Streptomyces achromogenes.
    • To explore the potential role of this enzyme in antibiotic biosynthesis.

    Main Methods:

    • Testing crude cell-free extracts of nine Streptomyces strains for nitroalkane oxidation.
    • Partial purification and characterization of the enzyme from Streptomyces achromogenes subsp. streptozoticus.
    • Enzyme kinetics and inhibition studies using various substrates and effectors.

    Main Results:

    • Crude extracts produced nitrous acid from several nitroalkanes, with varying efficiency among strains.
    • A partially purified enzyme from S. achromogenes oxidized 2-nitropropane to acetone and nitrous acid.
    • The enzyme was inhibited by reducing agents and thiol compounds but not by cyanide; sodium peroxide stimulated activity.

    Conclusions:

    • A novel nitroalkane-oxidizing enzyme from Streptomyces achromogenes was identified and characterized.
    • The enzyme's activity and substrate specificity were elucidated.
    • The enzyme may be involved in the biosynthesis of antibiotics containing a nitrogen-nitrogen bond.

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