An indirect electrochemical method for aqueous sulfide determination in freshwaters using a palladium chelate as a...

Sulfide anion is a highly toxic and corrosive compound and its presence above the threshold concentrations (i.e. μmol L^-1) in freshwaters may indicate environmental pollution. Besides, the increase in sulfide concentration results in modifications of the organoleptic proprieties of water and air. Many analytical methodologies have been designed for aqueous sulfide quantification, however, due to the high reactivity and instability of sulfide, the pursue of a simple, sensitive, selective, and portable analytical method is still a current demand. In this study, an indirect electrochemical method for the determination of sulfide based on its interaction with a palladium complex - bis(2-aminobenzoate) palladium(II) - acting as a selective chemosensor is described. The reaction leads to the demasking of the electroactive ligand 2-aminobenzoic acid (i.e. anthranilic acid) and square wave voltammetry is employed to monitor its concentration using a glassy carbon electrode (GCE). Experimental conditions were optimized and the reaction was performed in Britton-Robinson (BR) buffer at pH 5 for 4 min, providing the higher magnitude of the analytical signal. A linear relation (r² > 0.99) from 3 to 30 μmol L^-1 of sulfide was obtained with a limit of detection of 0.10 μmol L^-1. Recovery experiments using freshwater samples spiked with sulfide revealed overall satisfactory results for the limit concentration levels permitted by regulatory agencies. Therefore, the proposed methodology shows advantages in terms of portability, selectivity, sensitivity, low-cost, and easiness-to-use enabling monitoring of sulfide in a variety of waters.