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In vitro interaction between glabridin and voriconazole against Aspergillus fumigatus isolates
Mojtaba Nabili1, Narges Aslani2, Tahereh Shokohi3
1Faculty of Medicine, Sari Branch, Islamic Azad University, Sari, Iran.
Background:
Voriconazole (VRC) is widely recommended as the first-line therapy for invasive aspergillosis. However, surveillance studies have demonstrated that there is an increase in the frequency of azole resistance among Aspergillus fumigates isolates. In recent years, more studies on effective synergisms between natural agents and antifungal drugs have been published.
Aims:
To evaluate the synergistic antifungal effect of glabridin (Gla) and VRC against A. fumigatus isolates.
Methods:
Potential interactions between Gla and VRC were studied by using a microdilution checkerboard method based on the CLSI reference technique. To assess the interaction of drugs the fractional inhibitory concentration index (FICI) was calculated based on the Loewe Additivity model.
Results:
The minimum inhibitory concentrations (MIC) obtained with Gla alone were relatively high (MIC50 16μg/ml). However, our results showed synergistic interaction between Gla and VRC against A. fumigatus strains, with FICI range values between 0.15 and 0.5.
Conclusions:
Synergistic activity of Gla and VRC against both VRC-sensitive and -resistant A. fumigatus isolates may lead to design new antifungal agents, especially for inhibiting those azole-resistant strains.
Insights
Glabridin (Gla) and voriconazole (VRC) show synergistic antifungal effects against Aspergillus fumigatus, offering a potential strategy to combat azole-resistant strains. This combination may lead to new treatments for invasive aspergillosis.
Area of Science:
- Mycology
- Pharmacology
- Infectious Diseases
Background:
- Voriconazole (VRC) is a primary treatment for invasive aspergillosis.
- Increasing azole resistance in Aspergillus fumigatus necessitates alternative therapeutic strategies.
- Research is exploring synergistic combinations of natural compounds and antifungal drugs.
Purpose of the Study:
- To investigate the potential synergistic antifungal effect of glabridin (Gla) and VRC against Aspergillus fumigatus.
- To assess the interaction between Gla and VRC in inhibiting fungal growth.
Main Methods:
- Microdilution checkerboard method following CLSI standards.
- Calculation of Fractional Inhibitory Concentration Index (FICI) using the Loewe Additivity model.
- Evaluation of drug interactions against Aspergillus fumigatus isolates.
Main Results:
- Glabridin (Gla) alone exhibited high minimum inhibitory concentrations (MIC50 ≥ 16μg/ml).
- A synergistic interaction was observed between Gla and VRC against A. fumigatus.
- Fractional Inhibitory Concentration Index (FICI) values ranged from 0.15 to 0.5, indicating synergy.
Conclusions:
- The combination of Gla and VRC demonstrates synergistic activity against both VRC-sensitive and VRC-resistant A. fumigatus strains.
- This synergistic effect provides a basis for developing novel antifungal agents.
- The findings are particularly relevant for combating azole-resistant Aspergillus species.
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