Differential splicing and alternative polyadenylation generates distinct NCAM transcripts and proteins in the mouse

The neural cell adhesion molecule (NCAM) exists in at least three different protein isoforms which are selectively expressed by different cell types and at different stages of development. They are encoded by four to five different transcripts that are derived from a single gene. Here we report the exon--intron structure of the 3' part of the mouse NCAM gene. This region contains six exons. The 5' exon is constitutively expressed in all four prominent size classes of NCAM mRNAs detected in the mouse brain. The second exon contains the poly(A) addition sites for the two smaller mRNAs of 5.2 and 2.9 kb which differ in the length of their 3' non-coding regions and seem both to encode NCAM-120. This second exon is absent in the largest 7.4 kb transcript which encodes NCAM-180; in the 6.7 kb mRNA, which appears to code for NCAM-140, the second and the fifth exon have been spliced out. This data explains how the prominent four transcripts and three protein isoforms of mouse NCAM are generated from a single gene. The alternatively spliced fifth exon is surrounded by inverted repeats potentially capable of secondary structure formation, that may sequester this exon in a loop.