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Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

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Engineering adipic acid metabolism in Pseudomonas putida.

Yannic S Ackermann¹, Wing-Jin Li², Leonie Op de Hipt¹

¹Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich, Jülich, Germany.

Metabolic Engineering

|May 9, 2021

Summary

This study engineered Pseudomonas putida for bio-upcycling plastic components like adipic acid. The modified bacteria efficiently convert plastic waste into valuable biodegradable materials.

Keywords:

Adipic acid Metabolic engineering Metabolic network Plastic upcycling Polyhydroxyalkanoate Pseudomonas putida

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A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Published on: October 2, 2012

Area of Science:

Microbial biotechnology
Synthetic biology
Bioremediation

Background:

Plastic waste poses a significant environmental challenge.
Traditional recycling methods are limited for contaminated polymer streams.
Adipic acid and similar dicarboxylates are common plastic building blocks.

Purpose of the Study:

To engineer Pseudomonas putida KT2440 for efficient dicarboxylate metabolism.
To enable the bio-upcycling of plastic waste into valuable products.
To develop a microbial platform for converting fossil-resource-based polymers into biodegradable materials.

Main Methods:

Heterologous expression of Acinetobacter baylyi dcaAKIJP genes in P. putida.
Genomic integration of dca genes for stable expression.
Adaptive laboratory evolution to optimize dicarboxylate utilization.
Genomic analysis to identify key metabolic pathways (e.g., paa gene cluster).
Genetic modification, including psrA disruption, for enhanced growth and function.

Main Results:

Engineered P. putida strains demonstrated efficient growth on adipate as a sole carbon source.
Adaptive evolution and genetic modifications (psrA disruption) were crucial for high-performance growth.
The engineered strain successfully metabolized other medium-chain-length dicarboxylates (suberate, sebacate).
Achieved a growth rate of 0.35 ± 0.01 h⁻¹ and biomass yield of 0.27 ± 0.00 gCDW gadipate⁻¹ on adipate.
Production of polyhydroxyalkanoates up to 25% of cell dry weight from adipate.

Conclusions:

Successful bio-upcycling of adipic acid and related dicarboxylates by engineered P. putida.
Demonstrated potential for converting fossil-based polymers into biodegradable materials.
The developed microbial platform offers a sustainable route for plastic waste valorization.