Related Experiment Video

Updated: Nov 6, 2025

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
07:49

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Published on: August 3, 2018

11.5K

LncRNA MIAT Mediates ox-LDL-Induced Endothelial Cell Injury Via miR-206/RAB22A Axis

Yu Gao1, Jianwei Yue2, Zhiliang Huang3

  • 1Department of Geriatrics, The Sixth Hospital of Wuhan, Wuhan, Hubei, China.

The Journal of Surgical Research
|May 9, 2021
PubMed
Abstract

Insights

Long non-coding RNA MIAT promotes atherosclerosis by damaging endothelial cells. MIAT knockdown protects against this injury by regulating the miR-206/RAB22A pathway, offering a potential therapeutic target for atherosclerosis.

Area of Science:

  • Molecular Biology
  • Cardiovascular Research
  • RNA Biology

Background:

  • Long non-coding RNA myocardial infarction associated transcript (MIAT) plays a role in atherosclerosis (AS).
  • The specific functions of MIAT in endothelial cell dysfunction remain unclear.

Purpose of the Study:

  • To investigate the role of MIAT in endothelial cell dysfunction induced by oxidized low-density lipoprotein (ox-LDL).
  • To elucidate the molecular mechanism involving microRNA-206 (miR-206) and Ras-related protein Rab-22A (RAB22A).

Main Methods:

  • Quantitative real-time polymerase chain reaction and western blot to assess gene and protein expression.
  • Cellular assays (viability, invasion, migration, apoptosis, EMT) to evaluate endothelial cell injury.
  • Dual-luciferase reporter and RNA immunoprecipitation assays to confirm molecular interactions.

Main Results:

  • MIAT expression was elevated in ox-LDL-treated human umbilical vein endothelial cells (HUVECs).
  • MIAT knockdown improved HUVEC viability, migration, invasion, and epithelial-mesenchymal transition (EMT) while reducing apoptosis and inflammation.
  • MIAT directly targeted miR-206, which in turn targeted RAB22A, establishing a regulatory axis.

Conclusions:

  • MIAT knockdown mitigates ox-LDL-induced HUVEC injury through the miR-206/RAB22A pathway.
  • This finding highlights MIAT's potential as a therapeutic target for atherosclerosis.
Keywords:
Endothelial cellMIATRAB22AmiR-206ox-LDL

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.8K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
22.8K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.3K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.0K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
8.2K
Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...
5.5K