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Oviductal Proteins Effect in Rooster Spermatic Cryopreservation
R Camarillo1, I Jimenez2, A Guzman3
1Mastery in the Animal Reproduction Biology, Metropolitan Autonomous University, San Rafael Atlixco, CDMX, Mexico.
Cryo Letters
|May 9, 2021
Summary
Hen uterus-vaginal protein extracts can prevent sperm capacitation during cryopreservation. This maintains rooster sperm viability and fertilizing capability, similar to fresh semen.
Area of Science:
- Reproductive Biology
- Animal Science
- Cryobiology
Background:
- Cryopreservation of spermatozoa can lead to capacitation, negatively impacting fertility.
- Maintaining sperm viability and fertilizing capacity after thawing is crucial for artificial insemination and breeding programs.
Purpose of the Study:
- To investigate the potential of uterus-vaginal union protein factors to inhibit sperm capacitation.
- To assess the effect of these proteins on maintaining rooster sperm viability and fertilizing capability post-cryopreservation.
Main Methods:
- Rooster spermatozoa were cryopreserved using Lake extender.
- Different concentrations of hen's uterus-vaginal junction protein were added to the extender.
- Spermatic viability, physiological condition, and in vivo fertilizing capability were evaluated.
Main Results:
- It was possible to induce spermatic decapacitation in vitro.
- Cryo-capacitation was successfully inhibited by the protein extracts.
- Fertility results were comparable to those achieved with fresh, non-cryopreserved semen.
Conclusions:
- Uterus-vaginal protein extracts effectively induce spermatic decapacitation in vitro.
- These protein factors show promise in preserving rooster sperm quality and fertility after cryopreservation.
