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Related Concept Videos

Bricks01:14

Bricks

212
Bricks, a fundamental building material, are crafted from fired clay and exhibit a range of shapes, sizes, and colors. The production process starts with extracting local clay or shale, which is then crushed, ground, and screened for a fine texture. The refined material is blended with water, creating a pliable mixture that can be formed into bricks using one of three processes: soft mud, dry press, or stiff mud methods.
Soft mud bricks are shaped in molds with high moisture content and can be...
212

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Production of Handmade Open Pulled Straw (OPS) Using Digital Heating Gun for the Vitrification Process.

S W Kim1, M M R Chowdhury2, Y G Ko3

  • 1Animal Genetic Resources Research Center, National Institute of Animal Science, Rural Development Administration (RDA), Jeonbuk, Republic of Korea. sungwoo@korea.kr.

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|May 9, 2021
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Summary
This summary is machine-generated.

Handmade open pulled straws (OPS) can be efficiently manufactured using a digital heating gun for vitrification. The 0.25 mL straw demonstrated higher production efficiency compared to the 0.5 mL straw.

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Area of Science:

  • Reproductive biology
  • Cryopreservation techniques
  • Biotechnology

Background:

  • Vitrification is the preferred method for cryopreserving oocytes and embryos, surpassing traditional slow freezing.
  • Developing efficient methods for preparing vitrification devices is crucial for successful cryopreservation.

Purpose of the Study:

  • To evaluate efficient manufacturing methods for handmade open pulled straws (OPS) using a digital heating gun.
  • To determine optimal heating parameters for OPS production for vitrification applications.

Main Methods:

  • Manufacturing of handmade OPS using 0.5 mL and 0.25 mL straws with a digital heating gun.
  • Varying heating times and temperatures to assess production efficiency and OPS inner diameter.
  • Analyzing production rates based on straw volume and heat settings.

Main Results:

  • Production efficiency of OPS varied significantly with heating time and temperature for both 0.5 mL and 0.25 mL straws.
  • The 0.25 mL straw achieved higher production efficiency (up to 95.2%) compared to the 0.5 mL straw (up to 90.5%) under optimized conditions.
  • The inner diameter of OPS could be adjusted (200-300 μm) to accommodate different gamete sizes, embryo stages, or cell lines.

Conclusions:

  • Handmade OPS can be efficiently produced using a digital heating gun, serving as a viable vitrification device.
  • The 0.25 mL straw offers superior production efficiency for handmade OPS manufacturing.
  • These findings support the use of digital heating guns for the routine production of OPS for cryopreserving gametes, embryos, and cell lines.