Dendrimer-assisted boronate affinity cellulose foams for the efficient and selective separation of glycoproteins

Surfaces engineered to identify and enrich glycoproteins are of considerable interest in the diagnostic and detection fields. A boronate affinity (BA) material was proposed as a potential candidate for the isolation of glycoproteins. However, this material has the disadvantages of low efficiency and non-degradability. Herein, a novel dendrimer-amplified BA cellulose foam (PEI-PBA-CF) was fabricated via a mild two-step approach. The as-prepared PEI-PBA-CF exhibited a rapid adsorption equilibrium rate (within 60 min) and outstanding adsorption capacity for horseradish peroxidase (537.4 mg g^-1) and ovalbumin (495.5 mg g^-1). Furthermore, competitive adsorption experiments demonstrated that PEI-PBA-CF could achieve selective separation and purification of glycoproteins from complex biological samples due to the synergistic effect of the improved BA capacity by the dendrimer and the well-interconnected porous structure of the biomass matrix. Consequently, these cellulose foams might present new application opportunities in analytical and biomedical fields.