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Related Experiment Video

Updated: Nov 6, 2025

Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain
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ID4 Is Required for Normal Ependymal Cell Development.

Brenda Rocamonde1, Vicente Herranz-Pérez2,3, Jose Manuel Garcia-Verdugo2

  • 1INSERM, CNRS, APHP, Institut du Cerveau - Paris Brain Institute (ICM), Sorbonne Université, Paris, France.

Frontiers in Neuroscience
|May 10, 2021
PubMed
Summary

The transcriptional regulator ID4 is crucial for ependymal cell development and maturation. ID4 deficiency in mice leads to altered brain ventricles, reduced ependymal cell numbers, and impaired cilia function.

Keywords:
ID4braindevelopmentependymal celltranscription factor

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Ependymal cells are vital for brain health, lining ventricles and regulating cerebrospinal fluid (CSF) flow.
  • Proper ependymal cell function, including coordinated cilia beating, is critical for neurogenesis and CSF dynamics.
  • Dysfunctions in ependymal cells are linked to disruptions in gene regulation by transcription factors.

Purpose of the Study:

  • To investigate the role of the transcriptional regulator ID4 in ependymal cell development and maturation.
  • To determine the impact of ID4 deficiency on ependymal cell cytoarchitecture, number, and ventricular size.
  • To explore the potential involvement of ID4 in motor cilia function.

Main Methods:

  • Utilizing a mouse model with targeted Id4 gene deficiency (Id4-deficient mice).
  • Analyzing ventricular cell cytoarchitecture and ependymal cell numbers in wild-type versus Id4-deficient mice.
  • Evaluating the effects of ID4 absence during embryonic development on ependymal cell maturation.

Main Results:

  • Id4-deficient mice exhibited altered ventricular cell cytoarchitecture.
  • A decreased number of ependymal cells and enlarged ventricles were observed in Id4-deficient mice.
  • Absence of ID4 during embryonic development led to reduced ependymal cell numbers and delayed maturation.

Conclusions:

  • The transcriptional regulator ID4 plays a significant role in ependymal cell development and maturation.
  • ID4 is essential for maintaining normal ependymal cell cytoarchitecture and ventricular size.
  • ID4 may be a key factor in regulating motor cilia function in ependymal cells, suggesting potential therapeutic targets.