Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Viruses with RNA Genomes

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Set1 promotes Notch-induced transcriptional activation.

Cellular and molecular life sciences : CMLS·2026

Same author

Chronic stress drives liver cancer by impairing the hepatic kynurenine pathway and immune surveillance.

Nature metabolism·2026

Same author

Sublethal executioner caspase activation in hepatocytes promotes liver regeneration through the JAK/STAT3 pathway.

PLoS biology·2025

Same author

Guggulsterone ameliorates psoriasis by inhibiting keratinocyte proliferation and inflammation through induction of miR-17 directly targeting JAK1 and STAT3.

Biochemical pharmacology·2025

Same author

Heterozygous deletion of <i>Cul4b</i> in female mice leads to ovulatory dysfunction and female infertility.

Genes & diseases·2024

Same author

CUL4B protects kidneys from acute injury by restraining p53/PAI-1 signaling.

Cell death & disease·2024

Search research articles

Related Experiment Video

Updated: Nov 6, 2025

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

Published on: December 21, 2019

CUL4B facilitates HBV replication by promoting HBx stabilization.

Haixia Shan^1,2, Bo Wang^1,3, Xiaodong Zhang¹

¹Key Laboratory for Experimental Teratology of the Ministry of Education, Key Laboratory of Infection and Immunity of Shandong Province and Department of Immunology, School of Basic Medical Sciences, Shandong University, Jinan 250012, China.

Cancer Biology & Medicine

|May 10, 2021

Summary

This summary is machine-generated.

Cullin 4B (CRL4B) enhances Hepatitis B virus (HBV) replication by stabilizing the HBx protein. This interaction inhibits HBx degradation, suggesting CRL4B as a potential therapeutic target for HBV infection.

Keywords:

CUL4B HBV HBx HCC ubiquitination

More Related Videos

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Published on: February 1, 2017

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Published on: November 7, 2018

Related Experiment Videos

Last Updated: Nov 6, 2025

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

Published on: December 21, 2019

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Published on: February 1, 2017

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Published on: November 7, 2018

Area of Science:

Hepatology
Virology
Molecular Biology

Background:

Hepatitis B virus (HBV) infection poses a significant global health challenge.
Mechanisms regulating HBV replication are not fully understood.
Cullin 4B-RING ubiquitin E3 ligase (CRL4B) plays a role in various cellular processes.

Purpose of the Study:

To elucidate the role of CUL4B in HBV infection.
To investigate how CUL4B influences HBV replication.
To determine the molecular interaction between CUL4B and HBV proteins.

Main Methods:

Utilized Cul4b transgenic and conditional knockout mice models.
Employed liver cell lines with CUL4B overexpression or knockdown.
Conducted immunoprecipitation, immunofluorescence, and cycloheximide chase assays.

Main Results:

CUL4B was found to promote HBV replication in vivo and in vitro.
CUL4B interacts with Hepatitis B virus X protein (HBx).
CUL4B stabilizes HBx by inhibiting its ubiquitination and proteasomal degradation, increasing HBV pgRNA levels.

Conclusions:

CUL4B enhances HBV replication through interaction with HBx, disrupting its degradation pathway.
CUL4B represents a potential therapeutic target for developing anti-HBV treatments.