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Quantification of Myocardial Mitochondrial Membrane Potential Using PET.

Matthieu Pelletier-Galarneau1, Felicitas J Detmer1, Yoann Petibon1

  • 1Gordon Center for Medical Imaging, Massachusetts General Hospital, Harvard Medical School, 125 Nashua St., Boston, MA, 02114, USA.

Current Cardiology Reports
|May 10, 2021
PubMed
Summary

This study introduces a new method for in vivo tissue membrane potential (ΔΨT) quantification, a proxy for mitochondrial membrane potential (ΔΨm). This technique offers a novel biomarker for cardiovascular diseases.

Keywords:
Heart failureMitochondriaMitochondrial membrane potentialPositron emission tomographyTissue membrane potentialTriphenylphosphonium

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Area of Science:

  • Biomedical imaging
  • Cardiovascular research
  • Mitochondrial function

Background:

  • Mitochondrial membrane potential (ΔΨm) is crucial for cellular energy production.
  • In vitro methods for ΔΨm measurement have limitations.
  • Tissue membrane potential (ΔΨT) serves as a valuable in vivo proxy for ΔΨm.

Purpose of the Study:

  • To present a novel method for in vivo quantification of tissue membrane potential (ΔΨT).
  • To review the origin and role of mitochondrial membrane potential (ΔΨm).
  • To highlight potential applications of myocardial ΔΨT imaging in cardiovascular diseases.

Main Methods:

  • Utilized radiolabeled lipophilic cations, similar to those used for in vitro ΔΨm measurement.
  • Employed positron emission tomography (PET) with appropriate compartment modeling.
  • Validated the technique in both animal models and human subjects.

Main Results:

  • Demonstrated the feasibility of in vivo myocardial ΔΨT quantification using PET.
  • Observed low variability in ΔΨT among healthy individuals, indicating sensitivity to pathological changes.
  • Established ΔΨT as a potential new biomarker for cardiovascular disease assessment.

Conclusions:

  • In vivo assessment of myocardial ΔΨT using PET is a feasible and promising technique.
  • This method provides a new tool for studying cardiovascular disease pathophysiology.
  • Myocardial ΔΨT imaging has the potential to serve as a biomarker for disease staging, prognosis, and therapeutic response.