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Related Concept Videos

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

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Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for...
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UV–Vis Spectrum01:30

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When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs light between the wavelengths of 180–400 nm range, the UV spectrum is obtained, and if it absorbs light in the 400–780 nm wavelength range, the visible spectrum is obtained.     
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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
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The Electromagnetic Spectrum01:24

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Electromagnetic waves are categorized according to their wavelengths and frequencies, giving the electromagnetic spectrum. These waves are classified as radio, infrared, ultraviolet, etc. Radio waves refer to electromagnetic radiation with wavelengths ranging from millimeters to kilometers. Radio waves are commonly used for audio communications (i.e., radios) and typically result from an alternating current in the wires of a broadcast antenna. They cover a broad wavelength range and are used...
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The Electromagnetic Spectrum02:37

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The electromagnetic spectrum consists of all the types of electromagnetic radiation arranged according to their frequency and wavelength. Each of the various colors of visible light has specific frequencies and wavelengths associated with them, and you can see that visible light makes up only a small portion of the electromagnetic spectrum. Because the technologies developed to work in various parts of the electromagnetic spectrum are different, for reasons of convenience and historical...
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Related Experiment Video

Updated: Nov 6, 2025

Effective Analysis of Human Exposure Conditions with Body-worn Dosimeters in the 2.4 GHz Band
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Universal Spectrum Explorer: A Standalone (Web-)Application for Cross-Resource Spectrum Comparison.

Tobias Schmidt1, Patroklos Samaras1, Viktoria Dorfer2

  • 1Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising 85354, Germany.

Journal of Proteome Research
|May 10, 2021
PubMed
Summary
This summary is machine-generated.

The Universal Spectrum Explorer (USE) is a new web tool for comparing mass spectrometry data. It allows users to visualize and analyze peptide spectra from various sources, enhancing proteomics research.

Keywords:
USIbioinformaticsspectrum comparisonuniversal spectrum identifiervisualizationweb tool

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Area of Science:

  • Proteomics
  • Bioinformatics
  • Mass Spectrometry

Background:

  • Analyzing and comparing mass spectrometry data is crucial for proteomics research.
  • Current tools often lack cross-resource compatibility and user-friendly visualization.
  • Standardized data access is needed for efficient spectral analysis.

Purpose of the Study:

  • To introduce the Universal Spectrum Explorer (USE), a novel web-based tool.
  • To enable cross-resource visualization and comparison of peptide mass spectra.
  • To facilitate integration with existing proteomics data repositories and analysis pipelines.

Main Methods:

  • Developed a web-based tool (USE) utilizing the IPSA framework.
  • Implemented data retrieval via manual upload, universal spectrum identifier (USI), and a new REST interface.
  • Integrated with ProteomicsDB for accessing ProteomeTools data and Prosit for predicted spectra.

Main Results:

  • The USE provides a platform for visualizing and comparing peptide spectra from diverse sources.
  • It supports data retrieval from multiple online repositories and custom inputs.
  • Annotated mirror spectrum plots are exportable as high-quality vector graphics.

Conclusions:

  • The Universal Spectrum Explorer (USE) offers a versatile solution for mass spectrometry data analysis.
  • Its design allows for local usage and integration into other web platforms.
  • USE enhances data accessibility and comparability in proteomics research.